RNA
Part:BBa_K5038005
Designed by: Qiyu Tan Group: iGEM24_CJUH-JLU-China (2024-08-24)
LIRA_input05
Usage and Biology
LIRA_input05 is the target to be detected by LIRA_H05, it will bind to the loop at the top of LIRA_H05, and then change its conformation so that the ribosome could bind to the RBS.
(Figure 1. LIRA_input05 binds to LIRA_H05)
Characterization
In our experiments, we used EGFP as the reporter gene and input05 as the target RNA, and inferred the opening of the hairpin through the fluorescence intensity. A detailed information is given in BBa_K5038020and BBa_K5038022.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |